MMLV RNase H- reverse transcriptase
The M-MLV Reverse Transcriptase RNase H- is a genetically modified M-MLV Reverse Transcriptase which exhibits RNA or DNA dependent DNA polymerase, but lacks RNase H activity. This enzyme can synthesize cDNA using oligo dT, random primers or a mixture of both (Ask us about random 15mers!).
Removal of the RNase H activity results in an increase of full-length cDNA products and has been shown to increase resistance against inhibutors.
- cDNA synthesis (conventional or real-time qPCR)
- microarray labelling
- RNA analysis by primer extension
: - M-MLV Reverse Transcriptase RNase H-
- 5 x RT Reaction buffer 1 (with MgCl2 and DTT)
0.25 M Tris-HCl, 0.5 M KCl, 30 mM MgCl2, 25 mM DTT
- 5 x RT Reaction buffer 2 (Mg2+ and DTT free)
0.25 M Tris-HCl, 0.5 M KCl
- 25 mM MgCl2
- 20 mM MnCl2
- 100 mM DTT
This buffer flexibility means that maximum efficiency buffers may be optimised for applications where standard
buffer components may prove inhibitory (e.g. DTT in some dye-based RT-qPCR).
STORAGE AND SHIPPING BUFFER:
50% glycerol (v/v), 50 mM Tris-HCl pH 8.0 at 25ºC, 100 mM NaCl, 5 mM DTT, 1 mM EDTA, 0.1% NP-40.
SHIPPING AND STORAGE CONDITIONS
Routine storage: -20ºC
Shipping at room temperature has no detrimental effects on the quality of this reverse transcriptase.