dnature was started in 2009 to provide consulting services for those developing PCR and real-time qPCR assays. In 2010 the company expanded to include the distribution of products in these fields.
Technical director of dnature, John Mackay, has over 15 years experience in developing PCR assays and has been developing and optimising real-time PCR assays for more than 10 of these. Over the past few years John has developed diagnostic qPCR assays and DNA/RNA extraction methods for a range of applications, including truffle speciation, wine spoilage yeast and bacteria quantification and plant pathogen detection. He has developed a number of multiplex qPCR assays, including the detection of 5 grapevine viruses plus internal control in the one reaction.
In 2008-9 he developed a novel duplex hydrolysis ('TaqMan') probe assay for Avocado Sunblotch Viroid (ASBVd) that was used to declare country of freedom for New Zealand, from this dangerous pathogen.
John also has a special interest in high resolution melting analysis and has designed a number of genotyping SNP assays using both amplicon and probe (unlabelled and snapback) formats. He has developed and published a DNA fingerprinting method using the high resolution melting of microsatellites and is currently collaborating with experts in the USA on extending the use of this application. John previously held a range of positions for Roche Applied Science and so brings a strong sales and technical support background to dnature.
The major drive of the testing applications for dnature is using DNA methods to help solve issues facing various industries (DNA in a range of nature = dnature). New qPCR applications continue to be developed - the most recent being rapid tests to differentiate the 2010 strains of kiwifruit Psa (Pseudomonas syringae pv. actinidiae). Further work is progressing. . .
MACKAY J.F. and Wittwer C.T. (2011). High Resolution Melting Analysis. In PCR Troubleshooting and Optimization: The Essential Guide. Horizon Press.
Gladding P., MACKAY J., Webster M., White H., Ellis K., Lee M., Kasabov N. & Stewart R. (2010). Longitudinal study of a 9p21.3 SNP using a national electronic healthcare database. Future Medicine 7(4): 361-369
Bell V.A., Bonfiglioli R.G., Walker J.T., Lo P.L., MACKAY J.F. and McGregor S.E. (2009). Grapevine Leafroll-associated Virus 3 Persistence in Vitis vinifera remnant roots. J. Plant Path. 91(3): 527-533
MACKAY J.F., Wright C.D., Bonfiglioli R.G. (2008). A new approach to varietal identification in plants by microsatellite high resolution melting analysis: application to the verification of grapevine and olive cultivars. Plant Methods. May 19;4:8.
Mackay I.M., MACKAYJ.F., Nissen M.D., and Sloots T.P. (2008). Real time PCR: History and Fluorogenic Chemistries. In Real-time PCR in Microbiology: from Diagnosis to Characterisation. Ed: Mackay I.M., Caister Academic Press, UK.
Beld M.G.H.M et al. (2008) Experts' roundtable: Real-time PCR and microbiology. In Real-time PCR in Microbiology: from Diagnosis to Characterisation. Ed: Mackay I.M., Caister Academic Press, UK.
MACKAY J., Landt O. (2007). Real-time PCR fluorescent chemistries. Methods Mol. Biol. 353:237-261.
MACKAY J. (2007). Introduction to Real-time (kinetic) PCR. Methods Mol. Biol. 353: 167-176