This PCR is extremely Wittwer

Posted by John on 28 January 2015 | 6 Comments

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OK, confession time. I used to work some crazy hours in order to fit some library screening into a part time job while finishing my degree. No, that's not the confession - more, when I put the PCR on I would sometimes look out the window and note the wind direction. Occasionally, very occasionally (*cough*) if the wind was blowing in the right direction and looked right, I would walk home  - paraglider in the car- drive out to the Otago Peinsula and if I was lucky soar above a beautiful beach for a couple of hours. Land, back it the car and 20 minutes later I'd be back in the lab to run the gel. Three to three and a half hours from putting on the PCR to running the gel.

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qPCR inventor and his NZ connection

Posted by John on 11 May 2014 | 3 Comments

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So you might have read the previous entry about the visit to see the first thermalcycler "Mr Cycle" at the Smithsonian. You haven't? No problem, I'll wait while you jump over to have a read . . . .
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I mentioned to Mallory (who kindly showed me the items) about the invention of qPCR and the original apparatus. Upon leaving, I contacted the person involved in those early days (including the photo of myself with Mr Cycle) and he replied back about how Mr Cycle had been on the bench next to him when he joined Cetus. Unfortunately the original qPCR apparatus had been disbanded - as he wrote "It was really just a light stand, a camera, a thermocycler, a light source and a PCR brought together"

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