killing that DNA

Posted by John on 11 October 2015 | 0 Comments

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1. You've done the hard work - you've got good quality RNA. You've seen ribosomal bands on a gel (or *cough* Fragment Analyzer). A good RQN/RIN (if that's what you go by) and everything's great.

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I have *what* in my sample?

Posted by John on 4 May 2015 | 1 Comments

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Reagent contamination in PCR has long been documented - not only by operators but also from commercial sources long before those operators ever laid DNA-ridden hands on them.

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This PCR is extremely Wittwer

Posted by John on 28 January 2015 | 6 Comments

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OK, confession time. I used to work some crazy hours in order to fit some library screening into a part time job while finishing my degree. No, that's not the confession - more, when I put the PCR on I would sometimes look out the window and note the wind direction. Occasionally, very occasionally (*cough*) if the wind was blowing in the right direction and looked right, I would walk home  - paraglider in the car- drive out to the Otago Peinsula and if I was lucky soar above a beautiful beach for a couple of hours. Land, back it the car and 20 minutes later I'd be back in the lab to run the gel. Three to three and a half hours from putting on the PCR to running the gel.

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