more more plasmid

Posted by John on 1 October 2010 | 0 Comments

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In the days when doing plasmid minipreps, I certainly enjoyed the increased yields from using Terrific Broth (TB) over standard LB. Although, because it took a while to make - and would get pinched by others and inevitably contaminated - it was always labelled as something else. UFB used to stay on the shelf quite happily. Indeed, if the recipe was for Terrific Broth then mine was better than just terrific - it was Un-%&$#ing-Believable.

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A fast way to speed up plasmid minipreps

Posted by John on 9 June 2010 | 1 Comments

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I put this tip out in our latest dnature newsletter. . .but couldn't find the reference to cite and thus included an offer to those who could use literature databases better than I.
However thanks to those who got in touch to say they already used the method (cheers Craig, Michael and Elena). I'm glad to know I didn't dream the thing.

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