qPCR inventor and his NZ connection

Posted by John on 11 May 2014 | 3 Comments

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So you might have read the previous entry about the visit to see the first thermalcycler "Mr Cycle" at the Smithsonian. You haven't? No problem, I'll wait while you jump over to have a read . . . .
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I mentioned to Mallory (who kindly showed me the items) about the invention of qPCR and the original apparatus. Upon leaving, I contacted the person involved in those early days (including the photo of myself with Mr Cycle) and he replied back about how Mr Cycle had been on the bench next to him when he joined Cetus. Unfortunately the original qPCR apparatus had been disbanded - as he wrote "It was really just a light stand, a camera, a thermocycler, a light source and a PCR brought together"

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Come in spinner

Posted by John on 14 April 2013 | 2 Comments

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That last little job before running your qPCR - no, not trying to remember which random sample you loaded in the wells; rather, centrifuging down the reactions to the bottom of the wells. This removes air bubbles and ensures all reaction components are combined.

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two better than one

Posted by John on 10 September 2012 | 6 Comments

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Working with RNA viruses (and multiple isolate strains), sometimes it can be hard enough to get sufficiently conserved sequence for primers (and harder if designing a TaqMan or probe-based assay). However a recent example in our lab showed the benefit of designing multiple assays for the same target and comparing the performance across template dilutions.

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being sensitive to qPCR

Posted by John on 22 April 2010 | 2 Comments

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This article was originally published at www.bitesizebio.com on 14/10/2009 here

One of the great features of PCR is its excellent sensitivity as we know. And many articles describe real-time qPCR as an added leap forward in that sensitivity – to the point where it has become a standard feature of a new assay description

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