The solution (of your primers)

Posted by John on 30 July 2010 | 0 Comments

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As PCR primers become less expensive, is there less care being paid to them? Throw in a diluent,a quick vortex and dilute for PCR?
I hope this doesn't sound too familiar because even in these days of real-time qPCR and various probe chemistries, your PCR primers still reign king in the reaction. Your probe design is not going to determine the efficiency and - usually - the sensitivity of your PCR and qPCR reactions. Therefore its worth paying a little care in looking after your PCR primers - especially if they're being used for a large number of runs and samples.

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