A fast way to speed up plasmid minipreps

Posted by John on 9 June 2010 | 1 Comments

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I put this tip out in our latest dnature newsletter. . .but couldn't find the reference to cite and thus included an offer to those who could use literature databases better than I.
However thanks to those who got in touch to say they already used the method (cheers Craig, Michael and Elena). I'm glad to know I didn't dream the thing.

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Getting the most off your PCR columns

Posted by John on 20 May 2010 | 0 Comments

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A quick tech tip I've found useful to maximise yield if using a low elution volume for column purification ie <50┬Ál. I usually use this for PCR reaction purification but it also works for genomic DNA and probably other extractions as well (e.g. plasmid and RNA) if you're using lower elution volumes than suggested.

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