cDNA synthesis
We offer a range of products from Quanta BioSciences to meet your cDNA application requirements - products designed for the specific applications in today's research, including qRT-PCR.
Product Comparison
| qScript™ cDNA Supermix | qScript™ cDNA Synthesis Kit | qScript™ Flex cDNA Kit |
|
|---|---|---|---|
| Real-Time qRT-PCR | Ideal for qRT-PCR | Ideal for qRT-PCR | qRT-PCR with flexible priming options |
| Amplicon length | ≤ 800bp | ≤ 1kb | ≤ 12kb (oligo-dT or gene specific primer) |
| Priming Options | Optimised mixture of oligo-dT and random primers | Optimised mixture of oligo dT and random primers | Choice of oligo-dT OR random primer OR gene specific primer |
| Convenience | Ultimate convenience
|
2 separate tubes
|
Similar ease of use as qScript cDNA Synthesis Kit but allows user defined priming method |
| Complexity of 1st strand cDNA |
Unbiased RNA sequence representation | Unbiased RNA sequence representation | Dependent on priming method |
| RNase Inhibitor | Blended with Reverse Transcriptase | Blended with Reverse Transcriptase | Blended with Reverse Transcriptase |
| RNA Input Range (linear dynamic range) |
10pg – 2µg total RNA | 1pg – 500ng total RNA | 10pg – 1µg total RNA |