Over the past few years John has developed diagnostic qPCR assays and DNA/RNA extraction methods for a range of applications, including truffle speciation, wine spoilage yeast and bacteria quantification and plant pathogen detection. He has developed a number of multiplex qPCR assays, including the detection of 5 grapevine viruses plus internal control in the one reaction. In 2008-9 he developed a novel duplex hydrolysis ('TaqMan') probe assay for Avocado Sunblotch Viroid (ASBVd) that was used to declare country of freedom for New Zealand, from this dangerous pathogen.

John also has a special interest in high resolution melting analysis and has designed a number of genotyping SNP assays using both amplicon and probe (unlabelled and snapback) formats. He developed and published a DNA fingerprinting method using the high resolution melting of microsatellites for determining plant cultivars - this method has now been extended to other plant and human microsatellite analyses by others.

The major drive of the testing applications for dnature is using DNA methods to help solve issues facing various industries (DNA in a range of nature = dnature).




Khan S., MACKAY J.F., Liefting L. and Ward L. (2015). Development of a duplex one-step RT-qPCR assay for the simultaneous detection of Apple scar skin viroid and plant RNA internal control. J. Virol. Methods 221: 100-105

Taking it to the Extreme: PCR at Wittwerspeed. MACKAY J.F. (2015). Clinical Chemistry 61(1): 4-5

Vanneste, J.L., Yu, J., Cornish, D.A., Tanner, D.J., Windner, R., Chapman, J.R., Taylor, R.K., MACKAY, J.F.,Dowlut. (2013). Identification, virulence and distribution of two populations of Pseudomonas syringae pv. actinidiae in New Zealand. Plant Disease 96 (6): 708-719

MACKAY J.F. and Wittwer C.T. (2011). High Resolution Melting Analysis. In PCR Troubleshooting and Optimization: The Essential Guide. Horizon Press.

Gladding​‌ P., MACKAY J​‌., Webster​‌ M., White​‌ H., Ellis​‌ K., Lee​‌ M., Kasabov​‌ N. & Stewart R. (2010).​ Longitudinal study of a 9p21.3 SNP using a national electronic healthcare database. Future Medicine 7(4): 361-369

Bell V.A., Bonfiglioli R.G., Walker J.T., Lo P.L., MACKAY J.F. and McGregor S.E. (2009). Grapevine Leafroll-associated Virus 3 Persistence in Vitis vinifera remnant roots. J. Plant Path. 91(3): 527-533

 MACKAY J.F., Wright C.D., Bonfiglioli R.G. (2008). A new approach to varietal identification in plants by microsatellite high resolution melting analysis: application to the verification of grapevine and olive cultivars. Plant Methods. May 19;4:8.

Mackay I.M., MACKAYJ.F., Nissen M.D., and Sloots T.P. (2008). Real time PCR: History and Fluorogenic Chemistries. In Real-time PCR in Microbiology: from Diagnosis to Characterisation. Ed: Mackay I.M., Caister Academic Press, UK.

Beld M.G.H.M et al. (2008) Experts' roundtable: Real-time PCR and microbiology. In Real-time PCR in Microbiology: from Diagnosis to Characterisation. Ed: Mackay I.M., Caister Academic Press, UK.

MACKAY J., Landt O. (2007). Real-time PCR fluorescent chemistries. Methods Mol. Biol. 353:237-261.

MACKAY J. (2007). Introduction to Real-time (kinetic) PCR. Methods Mol. Biol. 353: 167-176


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