Presto™ Mini gDNA Bacteria Kit 4 Preps

The Presto™ Mini gDNA Bacteria Kit is a Bacteria genomic DNA extraction kit optimized for genomic and viral DNA purification from Gram negative bacterial cells and Gram positive bacterial cells. This Bacteria Genomic DNA Extraction Kit includes Gram+ Buffer and Lysozyme and when combined, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Chaotropic salt is used to lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the genomic DNA spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 30 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA, with approximately 20-40 kb, is suitable for use in PCR or other enzymatic reactions.

Features & Benefits

• Purify genomic DNA within 30 minutes!
• High Yield: up to 40 μg (1 x 10⁹ Escherichia coli) and 15 µg (1 x 10⁹ Bacillus subtilis)
• High Purity gDNA: A260/A280 >1.9
• DNA Size: 20-40 kb
• Samples: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 1 x 10⁹), 200 µl of blood and biological fluids such as plasma and urine
• Convenient: Includes Gram+ Buffer for preparing Lysozyme solutions to speed up sample preparation when purifying DNA from Gram (+) positive bacteria
• Spin Columns: glass fiber membrane optimized for genomic DNA extraction (sterilised to remove bacteria contamination)
• Elution volume: 30-200 µl
• Storage: dry at room temperature (15-25°C)

Applications

PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions

Components

• Proteinase K
• Gram+ Buffer
• GT Buffer
• GB Buffer
• W1 Buffer
• Wash Buffer
• Lysozyme
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
• GD Columns
• 2 ml Collection Tubes

Full protocol

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