Isothermal

Isothermal amplification methods are alternatives to PCR. The LAMP (loop-mediated isothermal amplification) reaction operates at a single temperature between 60-70 degrees and uses multiple primers to produce multiple DNA

Another isothermal amplification method is RPA (Recombinase Polymerase Amplification). This uses two longer PCR-type primers and amplicons can be detected via fluorescence, gel or other methods. It has the advantage of working at about 37 degrees – and even down to room temp.

At dnature, as well as supplying these reagents, we have experience in developing both LAMP and RPA reactions for our projects in the lab as well as for customer applications.