5x HOT FIREPol SolisGreen qPCR mix, 1 ml

New from Solis BioDyne, this Dye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate the DNA amplification process during PCR. HOT FIREPol^® SolisGreen^® qPCR Mix is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating SolisGreen^® dye.

It comprises all the components necessary, excluding the template and primers, to perform highly sensitive qPCR. The user simply needs to add water, template, and primers.

HOT FIREPol^® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Features & Benefits

• improved detection of low target concentrations
• higher fluorescence level
• reaction set-up and shipment without dry ice

Applications

• detection and quantification of DNA and cDNA targets
• profiling gene expression
• microbial detection
• viral load determination

Mix Components

• HOT FIREPol^® DNA Polymerase
• 5x qPCR buffer with 12.5 mM MgCl₂ 
• 1x PCR solution – 2.5 mM
• dNTPs blend containing dUTP/dTTP
• SolisGreen^® dye
• Internal reference based on ROX dye

Shipping & Storage

Routine storage: -20°C

Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product.

Full Protocol