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5X HOT FIREPol SolisGreen qPCR mix
New from Solis BioDyne, this Dye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate the DNA amplification process during PCR. HOT FIREPol® SolisGreen® qPCR Mix is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating SolisGreen® dye.
It comprises all the components necessary, excluding the template and primers, to perform highly sensitive qPCR. The user simply needs to add water, template, and primers.
HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Routine storage: -20°C
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product.