AccuStart II PCR ToughMix®, 4000 x 25 µl reactions

AccuStart II PCR ToughMix® is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. The only user supplied components are DNA template and molecular grade water.

A key component to AccuStart PCR ToughMix® is an ultra pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. These antibodies bind the polymerase and keep it inactive prior to the initial PCR denaturation step. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Similar to Taq DNA polymerase, the activated polymerase in AccuStart II PCR ToughMix® possesses 5′>3′ DNA polymerase activity and a double-strand specific 5′>3′ exonuclease. The polymerase does not have 3′-exonuclease activity and is free of any contaminating endo or exonuclease activities. PCR products generally contain non-templated dA additions and can be cloned using vectors that have a single 3′-overhanging thymine residue on each end.

GelTrack Loading Dye is a mixture of blue and yellow electrophoresis-tracking dyes that migrate at approximately 4 kb and 50 bp. This optional component simplifies post-PCR analysis with gel electrophoresis and eliminates potential for cross contamination by enabling direct transfer of PCR products to the gel sample wells.

Features & Benefits

• Stabilized 2X PCR SuperMix enables convenient room-temperature setup and is unaffected by repetitive freeze-thaw (>20X).
• High-yielding, ultrapure modified Taq DNA polymerase delivers robust, reliable duplex assay performance.
• Stringent, ultrapure antibody hotstart ensures sensitive and specific target amplification.
• Separate electrophoretic mobility dye reduces risk of post-PCR cross contamination with gel electrophoresis.

Components

• 2X AccuStart II PCR ToughMix®
• GelTrack Loading Dye

Shipping & Storage

Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.
Repeated freezing and thawing does not impair product performance.

Full Protocol

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Toughmix product information