CircLigase™ II ssDNA Ligase, 1,000 U

CircLigase™ II ssDNA Ligase* is a thermostable enzyme that catalyses intramolecular ligation (i.e. circularisation) of ssDNA templates having a 5´-phosphate and a 3´-hydroxyl group. In contrast to T4 DNA Ligase and Ampligase™ DNA Ligase, which ligate DNA ends that are annealed adjacent to each other on a complementary DNA sequence, CircLigase II ssDNA Ligase ligates ends of ssDNA in the absence of a complementary sequence. The enzyme is therefore useful for making circular ssDNA molecules from linear ssDNA. Circular ssDNA molecules can be used as substrates for rolling-circle replication or rolling-circle transcription..

Features

• Intramolecular ligation of single-stranded DNA ends containing 5′-phosphates and 3′-hydroxyl groups
• ATP-independent, non-catalytic enzyme
• Circularizes ssDNA >15 bases long
• Compatible with high temperature ligation reactions due to thermostability of CircLigase™ II enzyme

Applications

• Production of ssDNA templates for rolling-circle replication or rolling-circle transcription experiments.
• Production of ssDNA templates for RNA polymerase and RNA polymerase inhibitor assays.

Full protocol