eQo 1-step ToughMix®, 100 reactions

95301-100

eQo 1-step ToughMix® is a new to market lyophilised reagent system for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes without the need for dry ice transportation.

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Product Details

eQo 1-step ToughMix® is a lyophilised reagent system for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes. It is supplied with a proprietary rehydration buffer that when combined with the lyophilized “eQo beads” produces a stabilized 4X concentrated master mix.

The kit includes thermolabile UDG for amplicon carryover elimination, an enhanced warm-start reverse transcriptase (RT) and all other required components for 1-step RT-qPCR except RNA template and primer/probe(s). The reaction chemistry has been optimised for inhibitor tolerance and delivers exceptional performance in either single-plex or highly demanding multiplex 1 step RT-qPCR formats.

Components

  • Master max pouch containing 1 tube of lypholised beads plus a dessicated pouch
  • Rehydration buffer

Storage & Handling

Prior to rehydration:

Store all components refrigerated at 2°C to 8°C or at ambient temperature (≤ 25°C) until kit expiration date. Do not open the internal silver Master Mix pouch until ready to rehydrate the lyophilised beads using the included buffer. See below for the rehydration protocol.

For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

 

After rehydration:

Store the 4X-concentrated 1-step ToughMix at -20°C (-25°C to -15°C). The rehydrated ToughMix is stable at this temperature and withstands up to 20 freeze-thaw cycles without compromised functional performance. After thawing, mix gently, but thoroughly by vortexing. Centrifuge briefly to collect contents before use.