EvaGreen® Plus dye, 20X in water (trial size), 1mL

EvaGreen® Plus Dye is an improved alternative to Biotium’s original EvaGreen® Dye. The dye retains many of the essential benefits of the original patented EvaGreen® Dye, but has an improved signal-to-noise ratio that is more advantageous for a variety of DNA detection applications, including qPCR, digital PCR, and LAMP.

• Lower background & higher signal compared to original EvaGreen® Dye
• Better match for common qPCR instrument excitation sources
• Compatible with qPCR & HRM®
• Higher signal to noise offers advantages for digital PCR and isothermal applications
• Directly visualise amplification products in gel

Improvements over the Original

Similar to the original EvaGreen® Dye, EvaGreen® Plus Dye is a green fluorescent nucleic acid dye that is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. EvaGreen® Plus Dye improves upon these properties with lower background fluorescence and increased brightness upon binding dsDNA. The dye has an excitation maximum that is a better fit for common qPCR instruments, resulting in improved sensitivity. As a result, EvaGreen® Plus Dye can give earlier Ct values and better signal discrimination. These properties offer potential advantages for digital PCR and isothermal amplification applications.

Why EvaGreen® Dye?

The unique properties of Biotium’s EvaGreen® dyes make them particularly useful in quantitative real-time PCR applications. Relative to other green dyes, such as SYBR® Green I, both EvaGreen® dyes are generally less inhibitory toward PCR and less likely to cause nonspecific amplification. Both EvaGreen® dyes enable direct visualisation of the PCR product on a gel using a UV transilluminator or blue light box without the need for another gel stain. It can also be used at a much higher dye concentration, resulting in more robust PCR signal.