Geneius™ Micro gDNA Extraction Kit 100 Preps

Geneius™ Micro gDNA Extraction Kit 100 Preps

GMB100

Provides an efficient method for purifying DNA (including genomic and mitochondrial DNA) from small volumes of whole blood, dried blood spots (Whatman® FTA® Cards), urine and saliva.

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Product Details

The Geneius™ Micro gDNA Extraction Kit provides an efficient method for purifying DNA (including genomic and mitochondrial DNA) from small volumes of whole blood, dried blood spots (Whatman® FTA® Cards), urine and saliva. This DNA Extraction Kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Carrier RNA is included with this DNA extraction kit to improve the efficiency of DNA binding to the spin column membrane. Contaminants are removed using W1 Buffer and Wash Buffer (containing ethanol). The purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed in as little as 20 minutes without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 300 ng from 6 mm dried blood spots.

Features & Benefits

Purify genomic DNA from small volume blood samples (1 µl), dried blood spots (Whatman® FTA® Cards), urine, saliva

  • in as little as 20 minutes
  • with high yield – 300 ng of pure genomic DNA from a 6 mm diameter circle cut out from Whatman® FTA® Cards (dried blood spots)  
  • High Purity gDNA: A260/A280 = 1.8-2.0
  • DNA Size: 20-30 kb
  • Spin Columns are glass fibre membrane optimized for genomic DNA extraction
  • Elution volume: 30-100 µl

Applications

Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions.

Contents

  • S1 Buffer
  • S2 Buffer
  • Carrier RNA
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • Proteinase K
  • GD Columns
  • 2 ml Collection Tubes

 

Real-time PCR data

Genomic DNA was extracted from blood samples (1–64 µl) using the Geneius™ Micro gDNA Extraction Kit. The purified DNA was eluted in 30 µl of Elution Buffer followed by a Real-time PCR assay performed with a 3 μl aliquot as template, primers (designed to amplify the ACTB gene), and Fast SYBR Green PCR Master Mix using the StepOnePlus™ Real-Time PCR system (Applied Biosystems). The results confirm the efficiency of DNA extraction from as low as 1 µl blood samples.

Storage

Dry at room temperature (15-25°C) for up to 18 months without showing any reduction in performance