Geneius™ Micro gDNA Extraction Kit 300 Preps

The Geneius™ Micro gDNA Extraction Kit provides an efficient method for purifying DNA (including genomic and mitochondrial DNA) from small volumes of whole blood, dried blood spots (Whatman® FTA® Cards), urine and saliva. This DNA Extraction Kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Carrier RNA is included with this DNA extraction kit to improve the efficiency of DNA binding to the spin column membrane. Contaminants are removed using W1 Buffer and Wash Buffer (containing ethanol). The purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed in as little as 20 minutes without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 300 ng from 6 mm dried blood spots.

Features & Benefits

• Purify genomic DNA from small volume blood samples (1 µl), dried blood spots (Whatman® FTA® Cards), urine, saliva in as little as 20 minutes
• with high yield – 300 ng of pure genomic DNA from a 6 mm diameter circle cut out from Whatman® FTA® Cards (dried blood spots)
• High Purity gDNA: A260/A280 = 1.8-2.0
• DNA Size: 20-30 kb
• Spin Columns are glass fibre membrane optimized for genomic DNA extraction
• Elution volume: 30-100 µl

Applications

Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions.

Contents

• S1 Buffer
• S2 Buffer
• Carrier RNA
• W1 Buffer
• Wash Buffer
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
• Proteinase K
• GD Columns
• 2 ml Collection Tubes

Storage

Dry at room temperature (15-25°C) for up to 18 months without showing any reduction in performance

Full protocol

Quick protocol