GENEzol™ TriRNA Bacteria Kit 100 Preps

The GENEzol™ TriRNA Bacteria Kit is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from bacteria samples. Bacterial cell walls are initially lysed using Lysozyme. The sample is then homogenized in GENEzol™ Reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.

Benefits

• Purify total RNA within 20 minutes without chloroform phase separation or isopropanol RNA precipitation
• Sample Volume: bacteria cells up to 1 x 10⁹
• Format: phenol, chlorform, guanidine isothiocyanate plus spin column system
• High quality RNA: A260/A280 >1.8, A260/A230 >1.8
• Storage: GENEzol™ Reagent should be stored at 2ºC to 25ºC for up to 9 months, Lysozyme should be stored at -20ºC for up to 9 months

Applications

cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting

Components

• GENEzol™ Reagent
• Pre-Wash Buffer
• Lysozyme
• Bacteria Lysis Buffer
• Wash Buffer
• RNase-free Water
• RB Columns
• 2 ml Collection Tubes

Full protocol