Presto™ Endotoxin Free Mini Plasmid Kit 100 Preps

The Presto™ Endotoxin Free Mini Plasmid Kit was designed for transfection grade plasmid DNA purification from 1-5 ml of cultured E. coli cells using silica-membrane spin columns. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. In addition, an efficient endotoxin removal step is integrated in the procedure and endotoxins are precipitated in a proprietary reagent. TrueBlue Lysis Buffer (an optional colour indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralisation. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 50 minutes. The purified endotoxin free plasmid DNA is ready for use in transfection, restriction enzyme digestion, ligation, PCR, and sequencing reactions.

Features & Benefits

• Endotoxin Free, Transfection Grade Plasmid DNA: <0.1 EU/µg of plasmid DNA
• Convenient: TrueBlue Lysis Buffer is an optional colour indicator included with the kit to reduce common handling errors, while ensuring efficient cell lysis, neutralisation and maximum plasmid product yield so you can feel confident with each and every plasmid prep
• Plasmid Size: 1-15 kb
• High Yield: up to 40 µg of transfection grade plasmid DNA
• Sample Volume: 1-5 ml of cultured bacterial cells
• Elution Volume: 30-100 µl
• Spin Columns: glass fibre membrane optimised for plasmid DNA purification

Shipping & Storage

Dry store at room temperature (15-25°C) for up to two years

Full protocol

Quick protocol