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PIF025
Use pre-packed anion-exchange resin columns for plasmid DNA purification from bacterial cultures.
These Geneaid Plasmid DNA Extraction Midi/Maxi Kits use pre-packed anion-exchange resin columns for plasmid DNA purification from bacterial cultures. Modified Alkaline Lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA in the crude lysate is bound to the column and the contaminants are removed with the PW Buffer. The purified plasmid DNA is eluted by a high salt buffer and then precipitated with Isopropanol for desalting. The entire procedure can be completed in 2 hours without ultracentrifuges, HPLC or other toxic reagents.
Plasmid DNA midi endotoxin removal kits and plasmid DNA maxi endotoxin removal kits are also available.
Please contact us regarding a sample for the most suitable kit
Dry store at room temperature (15-25°C) for up to two years