Quantabio PerfeCTa™ SYBR® Green FastMix™, Low ROX™, 5000 x 20 µl reactions

PerfeCTa™ SYBR® Green FastMix™, Low ROX™ is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and DNA template. This rigorously optimized master mix contains of proprietary buffer technology, stabilizers and AccuFast™ Taq DNA polymerase to deliver maximum assay precision, sensitivity, and PCR efficiency for accelerated or conventional thermal cycling conditions for SYBR Green detection. Dye-based detection methods are critically dependent on highly specific amplification because dsDNA dyes will bind to any amplicon, including off-target primer elongation and primer dimerization. AccuFast™ hot start Taq DNA polymerase contains a proprietary mixture of ultra-pure monoclonal antibodies that stringently suppress primer elongation prior to the initial PCR denaturation step and allows for setup and multi-day storage at ambient room temperature prior to thermal cycling. AccuFast™ provides rapid release of fully active enzyme to support accelerated thermal cycling conditions.

Features & Benefits

• 2x concentrated reagents minimize pipetting steps, simplify reaction assembly and improve accuracy
• Superior assay sensitivity and specificity with ultrapure AccuStart enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hot start
• Supports efficient vortex mixing with proprietary anti-foaming formulation
• FastMix formulation supports both fast and standard thermal cycling conditions

Components

Single-tube, 2X concentrated reagent containing:

• 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP,
  dCTP, dGTP, dTTP)
• AccuFast Taq DNA Polymerase
• SYBR Green I dye
• ROX Reference Dye (for 580-585 nm excitation), and stabilizers.

Shipping & Storage

Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.
After thawing, mix thoroughly before using.

Full Protocol

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