Quantabio qScript Ultra SuperMix, 25 x 20 µl reactions

qScript Ultra SuperMix is a highly stabilised, efficient and easy-to-use single tube master mix for the synthesis of first-strand cDNA to reverse transcribe RNA to cDNA.

A key component is a novel, state-of-the-art, RNase H deficient reverse transcriptase that was engineered for improved thermo-stability, velocity, processivity, and resistance to many common inhibitors. qScript Ultra SuperMix contains all required components for first-strand cDNA synthesis except RNA template and is directly compatible with downstream 2-step RT-qPCR or RT-PCR procedures.

This mix has:

• Superior speed – 10 minute reaction time with a total protocol time of 13 minutes
• Enhanced thermo-stability – engineered for RT activity up to 65°C, overcoming challenging secondary structures
• Maximum yield & sensitivity – wide dynamic range with no loss in cDNA abundance linearity from total RNA 2.5 µg – 1 pg
• Ultimate inhibitor resistance – overcome a wide array of PCR inhibitors such as salt, heparin, melanin etc
• Balanced coverage of long transcripts – unbiased representation of the transcriptome in cDNA product
• Ease of use – single tube mix with all required components for cDNA synthesis except RNA template

Storage & Handling

Store components in a constant temperature freezer at -25°C to -15°C.  After thawing, mix thoroughly before use.

qScript Ultra Supermix protocol

qScript Ultra Supermix product flyer