Stay up to date
Subscribe to our newsletter to stay updated on our latest insights, products and promotions. We promise not to clog up your in-box.
Faster, better, tougher next generation cDNA SuperMix for first strand cDNA synthesis.
qScript Ultra SuperMix is a highly stabilised, efficient and easy-to-use single tube master mix for the synthesis of first-strand cDNA to reverse transcribe RNA to cDNA.
A key component is a novel, state-of-the-art, RNase H deficient reverse transcriptase that was engineered for improved thermo-stability, velocity, processivity, and resistance to many common inhibitors. qScript Ultra SuperMix contains all required components for first-strand cDNA synthesis except RNA template and is directly compatible with downstream 2-step RT-qPCR or RT-PCR procedures.
This mix has:
Store components in a constant temperature freezer at -25°C to -15°C. After thawing, mix thoroughly before use.
We found that the qScript Ultra Supermix was great.
I directly compared Ultra (95217) with what we had currently been using (q Script, 95048).
We found that the Ultra reagent was superior.
I feel like it’s easier to explain with a picture.
We used three different primer sets (A, B, C) with two different samples (1 & 2).
For set A results were similar enough between reagents. However, for set B & C the product yield was greater with Ultra, especially for sample 1. Unfortunately my positive control did work well in that run for B & C.
I think we would have saved a lot of time and money with downstream sequencing had we been able to use Ultra for the whole project, and hopefully we can use this going forward. SB – Massey University