RNA Cleanup Kit, 50 Preps

The RNA Cleanup Kit uses a simple and efficient spin column procedure to purify total RNA stored in RNase-free water, elution buffer or TE Buffer following extraction using acid-guanidinium-phenol-chlorofom based methods such as TRIzol® Reagent and GENEzol™ Reagent. Contaminants such as RNases, DNA and residual phenol are effectively removed using a simple 4 step procedure. The high-quality, total RNA is eluted in RNase-free Water or TE (RNase-free) and is ready for use in a variety of sensitive downstream applications.

Features & Benefits

• Purify up to 50 µg of total RNA in 10 minutes
• Compatibility: purify RNA stored in RNase-free water, elution buffer or TE buffer following RNA extraction using GENEzol™, TRI-Reagent®, TRIzol®, RNAzol®, QIAzol® etc.
• Sample Volume: up to 100 μl of RNA products
• Membrane: glass fiber membrane optimized for total RNA extraction
• Spin Columns: individually packaged RNA spin columns, certified RNase and DNase-free
• Elution Volume: 20-50 μl
• Recovery: up to 80% of high-quality RNA (A260/A280 = 1.9-2.0)

Applications

RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, cDNA Synthesis, RNase Protention Assay

Contents

• RNA Pure Buffer
• Wash Buffer
• RNase-free Water
• PR Columns
• 2 ml Collection Tubes

Storage

Store at room temperature (15-25ºC)

Full protocol