SoliSD™ Lyo-compatible Bsm DNA Polymerase Kit, 100 reactions (sample)

Exceptionally stable Glycerol-Free SoliSD™ Bsm DNA Polymerase for isothermal amplification

SoliSD™ Lyo-compatible Bsm DNA Polymerase Kit is provided in a flexible 5-vial format. Implemented Stability TAG and the unique SoliSD™ Supplement system improves the performance of the enzyme:

• Glycerol free format – compatible with lyophilisation
• Stable for at least 1 month at room temperature
• No NTC signal
• Fast time to result from 4 minutes
• Strong strand displacement activity
• Active at 51-62°C with optimum at 60°C
• Lyo-compatible RT-LAMP Kit available upon request

SoliSD™ Glycerol-Free Bsm DNA Polymerase is based on the Large Fragment of the DNA Polymerase from the genus Bacillus smithii with functional similarity to other Bst and Bsm DNA polymerases. Implemented Stability TAG technology [1] ensures incredible stability for at least 1 month at 37°C.

The unique SoliSD™ Supplement system is provided with the kit. Technology improves the performance of the SoliSD™ Bsm DNA Polymerase:

• Resolves common NTC signal issue
• Minimizes variability between replicates
• Allows reaction set-up at room temperature

The enzyme has a strong strand displacement DNA polymerase activity and lacks 5’→3’ and 3’→5’ exonuclease activities. It is designed for applications requiring synthesis through double-stranded DNA regions, such as loop-mediated isothermal amplification (LAMP).

Contents

• SoliSD™ Glycerol-Free Bsm DNA Polymerase (40 U/μl)
• 10x Isothermal Reaction Buffer (pH 8.8) 200 mM Tris-HCl, 150 mM (NH₄)₂SO₄, 1% Tween-20, 250 mM KCl
• 100 mM MgSO₄
• 25x SoliSD Supplement
• 10x GC-rich Enhancer
• NB: dNTPs are required separately – order here

Storage & Shipping

Routine storage at -20 °C and shipping at room temperature

SoliSD™ Lyo-compatible Bsm DNA Polymerase Kit Protocol

SoliSD™ Lyo-compatible Bsm DNA Polymerase Kit Product Flyer

Certificate of Analysis