SolisFAST® Master Mix, (1 mL) 250 x 20 µL reactions

SolisFAST^® Master Mix is a 5x-concentrated, ready-to-use solution for fast singleplex and multiplex endpoint PCR assays. The mix allows amplification of up to 5 kb fragments from low complexity DNA templates (e.g. lambda, plasmid DNA), and up to 3 kb from complex DNA templates (e.g. human genomic DNA).

Combining a novel in-silico designed SolisFAST^® DNA Polymerase and optimised buffer, this mix offers robust PCR, accurate target detection, ice-free shipping and reaction set-up.

Features & Benefits

• Fast – less time from sample to results
• Accurate – reliable results in single- and multiplex assays
• Stable – 30-day stability at room temperature
• Trustworthy – avoid carry-over contamination with UNG mixes and get reliable results in Sanger sequencing applications
• PCR results in 20 minutes
• reliable results in single and multiplex reactions
• stable for 30 days at room temperature
• robust performance
• suitable Sanger sequencing applications

Mix Components

• SolisFAST^® DNA Polymerase
• PCR buffer, 17.5 mM MgCl₂ 
• 1x PCR solution 3.5 mM MgCl₂

Shipping & Storage

Routine storage: -20°C

Shipping and temporary storage for up to 1 month at room temperature or storage for up to 6 months at +4°C  has no detrimental effects on the quality of SolisFAST^® Master Mix.

Full Protocol

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