Total RNA Mini Kit (Plant)

RP100

An RNA Isolation Kit designed for total RNA purification from plant tissue & cells.

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Product Details

This kit is an RNA Isolation Kit designed for RNA purification from plant tissue & cells using an efficient RNA miniprep system.

The RNA kit will purify RNA from a wide variety of plant species within 15 minutes.  Samples are initially ground in liquid nitrogen then filtered to remove cell debris using the efficient RNA filter columns in the kit.  In the presence of a binding buffer and chaotropic salt, total RNA in the lysate binds to the glass fibre matrix of the RNA spin column.  Once any contaminants have been removed using the wash buffer (that contains ethanol), the purified total RNA is eluted by RNase-free water.  Phenol extraction or alcohol precipitation is not required and the purified total RNA is ready for use in real time PCR, RT-PCR and Northern Blotting.

Each Geneaid RNA kit includes individually packaged certified DNase and RNase-free spin columns & collection tubes.

 

Features & Benefits

  • Fast – able to purify total RNA within 15 minutes
  • Provides high yield – 5-30ug of pure RNA
  • Sample size – up to 100mg (fresh plant tissue) and up to 25mg of dry plant tissue
  • Glass fibre membrane of spin columns has been optimised for total RNA extraction
  • RNA spin columns & collection tubes are individually packaged and are certified RNase & DNase-free

 

Applications

  • RT-PCR
  • Northern Blotting
  • Real-time PCR

 

Components

  • RB Buffer
  • PRB Buffer
  • W1 Buffer
  • Wash Buffer
  • RNase-free Water
  • Filter Columns
  • RB Columns
  • 2ml Collection Tubes

Extracted RNA from freeze dried plant material (ELISA positive controls) in 2017. Even now, in 2023, that RNA is still fine to be used as positive PCR control using either the Quantabio UltraPlex® 1-Step ToughMix or the Quanta qScript™ XLT One-Step RT-PCR Kit. Impressive! JM, private laboratory