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Total RNA Mini Kit (Tissue) 300 Preps
RT300
Is a RNA Isolation Kit designed for total RNA purification from a variety of animal tissue using an efficient RNA miniprep system.
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Product Details
The Total RNA Mini Kit (Tissue) is a RNA Isolation Kit designed for total RNA purification from a variety of animal tissue using an efficient RNA miniprep system. This RNA Kit will purify RNA from tissue samples within 15 minutes. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided DNase and RNase-free Micropestle. The Total RNA Mini Kit (Tissue) uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required and the purified RNA is ready for use in a variety of downstream applications. Each Geneaid RNA Kit icludes individually packaged certified DNase and RNase-free spin columns and collection tubes.
Features & Benefits
- Purify total RNA within 15 minutes
- Sample – up to 25 mg of tissue (tailsnips, liver, kidney, brain, spleen, earpunches etc)
- High Yield – 5-30 μg of pure RNA
- RNase and DNase-free Micropestles included for simple tissue homogenization within the tube to reduce the risk of sample contamination
- Spin Columns – glass fibre membrane optimized for total RNA extraction
- Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
Components
- RB Buffer
- W1 Buffer
- Wash Buffer
- RNase-free Water
- RB Columns
- Filter Columns
- 2 ml Collection Tubes
- Micropestles
RNA Mini Kit (Tissue) Functional Test Data
Specifications | |||||||
| Cat. # | RB050 RB100 RB300 | RT050 RT100 RT300 | RP050 RP100 RP300 | RBB050 RBB100 RBB300 | RBY050 RBY100 RBY300 | RMI050 | GZX050 GZX100 GZX200 |
Format | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Tri Reagent Spin Column |
| Binding Capacity | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg |
| Sample | blood, cells | animal tissue | plant tissue | bacterial cells | yeast cells | animal tissue, cells | blood,cells, tissue, body fluids, bacteria |
| Micropestle | N/A | Yes | N/A | N/A | N/A | Yes | N/A |
| Filter Column | N/A | Yes | Yes | N/A | N/A | N/A | N/A |
| Enzyme | N/A | N/A | N/A | Lysozyme | N/A | N/A | N/A |
Typical Yield | 2-3 µg from 300 µl blood, 25-30 µg from 106 cells | 5-30 µg | 5-30 µg | 40-50 µg from 1×109 bacterial cells | 15-20 µg from 5×107 yeast cells | 5-30 µg | 5-30 µg |
| Elution Volume | 50 µl | 50 µl | 50 µl | 50 µl | 50 µl | 50 µl | 25-50 µl |
| Time | <20 min. | <15 min. | <20 min. | <20 min. | <20 min. | <30 min. | <15 min. |
