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a next-generation tool for first-strand cDNA synthesis, easy-to-use solution for two-step RT-PCR and RT-qPCR
qScript™ XLT cDNA SuperMix is a next-generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy-to-use solution for two-step RT-PCR and RT-qPCR. qScript™ XLT is an engineered M-MLV reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than cDNA synthesis kits utilizing an RNase H(+) reverse transcriptase (RT).
Single-tube, 5X concentrated reagent containing:
Store components in a constant temperature freezer at -25°C to -15°C upon receipt.
After thawing, mix thoroughly (vortex!) before using briefly spin-down to collect contents before opening tube. 5X concentrated reagent is viscous. When drawing out material, touch pipette tip to liquid interface but do not immerse.