Using the BMS Myra for repetitive pipetting …
October 30, 2022
Recently we’ve had a project in our lab to test several hundred samples for a range of viruses – all using one step RT-qPCR to test for the presence and quantify the levels of each target.
We used the Myra pipetting robot to make the job easier once we had the RNA extracted. Samples were transferred from individual extraction tubes to 96 well plates, 8 at a time, using the expanding tip spacing feature of the Integra Voyager pipettes. While the Myra easily handles eppies, the sample numbers made for easier handling with 96 well plates.
We could have had the Myra assemble the working mastermix for us as we do with our pre-formatted kits, however in this instance we prepared the hydrolysis probe mix and placed it on the deck. Dragging the samples across to the 96 well plate on the Myra deck then saving the whole set-up (sample names and all) as a template.
For each virus, we prepared the one tube of mastermix with specific primers and probes for that assay (virus and internal control). While the Myra can easily do this based on required primer, probe and mastermix volumes in the assay we prepared each one ahead of time using the Quantabio Virus 1-step.
Mastermix was rapidly dispensed to the Mic tubes and the templates from the 96-well plate loaded on top. The plates were capped in one step with the Rapid Capping tool and transferred to the two Mic qPCR Cyclers (multiple units can connect wirelessly to one PCR). While sample loading on the Myra can be easily exported for other qPCR instruments, sample names and loading information travel seamlessly across to the Mic runs so there’s no other information to enter once the run starts. The analysis (cycling data and Cq’s) has been automatically added in the assay so once the run has finished, the analysis is performed automatically.
Here we loaded the multiple plates using the project function on the Mic (can take hundreds of samples for absolute or relative quantification). Rather than rely solely on the Cq between run calibrators, the Project function uses the latest fluorescent algorithms based on the Factor qPCR work of Jan Ruitjer.
The beauty of having the whole set up saved as a template? Remove the used virus mastermix tube, add a new freshly-made one , replace qPCR tube racks and get the next one set up while the other qPCRs are running. All the sample data and preparation information remains the same.
For more on the Myra pipetting robot: https://vimeo.com/551265581
