Presto™ Mini gDNA Yeast Kit 4 Preps

Features

• Purify genomic DNA from (up to 2 x 10⁸) yeast and other fungus species using a simple and gentle 3 buffer system
• Simple and gentle reagent DNA precipitation method
• Convenient premixed Sorbitol Buffer
• Elution volume –  50-100 µl
• Time – 40 minutes

Description

The Presto™ Mini gDNA Yeast Kit offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial or viral DNA from Saccharomyces cerevisiae and a variety of other yeast and fungus species. This highly versatile solution based system offers a convenient procedure with minimal hands on time. The provided Sorbitol Buffer, when combined with zymolase or lyticase, will efficiently lyse yeast and other fungus species cell walls consisting of chitin and polysaccharides. Purified DNA (A260/A280 = 1.8-2.0), is suitable for use in PCR or other enzymatic reactions.

Contents

• Sorbitol Buffer
• Cell Lysis Buffer
• Protein Removal Buffer
• DNA Hydration Buffer

Storage

Dry at room temperature (15-25°C) for up to 2 years without showing any reduction in performance

Full protocol